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KMID : 0525720170220040248
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Journal of Chitin and Chitosan
2017 Volume.22 No. 4 p.248 ~ p.253
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Anti-inflammatory Effects of Ecklonia cava by the Regulation of TSLP in TNF-α/IFN-γ-Stimulated Keratinocytes
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Kang Na-lae
Ahn Gin-Nae
Han Eui-Jeong
Shin Eun-Ji
Han Hee-Jin
Jeon You-Jin
Ahn Chang-Bum
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Abstract
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Human thymic stromal lymphopoietin (TSLP), secreted from keratinocytes, activates the inflammatory cytokines and directly stimulates the sensory neurons triggering scratching behavior, and finally leads to the development of skin inflammatory diseases such as atopic dermatitis. In this study, we verified the anti-inflammatory effects of Ecklonia cava ethanol extracts (ECE) through TSLP down-regulation in tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced inflammation in HaCaT, a human keratinocytes. Also, we observed the change of scratching behavior of mice with atopic dermatitis after the oral administration of ECE. First, ECE reduced the mRNA expression and secretion levels of TSLP in TNF-α/IFN-γ-stimulated HaCaT keratinocytes. Also, ECE inhibited the mRNA expression or secretion levels of inflammatory cytokines such as Interleukin (IL)-4, -5, -13, and TNF- α as well as the mRNA expression level of thymus and activation regulated chemokine (TARC), a chemokine. Moreover, ECE significantly reduced the scratching frequency in NC/Nga mice with spontaneous atopy diseases. According to these results, ECE has the anti-inflammatory effect and a probability as a natural materials for the prevention of skin diseases.
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KEYWORD
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Ecklonia cava ethanol extracts (ECE), Thymic stromal lymphopoietin (TSLP), HaCaT keratinocytes, NC/Nga, Skin inflammation
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